Knowledge and understanding: to acquire a basic knowledge of fundamental principles of most common spectroscopic methods of analysis, and of the general components of spectroscopic instruments. To know the most useful applications of spectroscopic methods, in particular to material science and mineralogy. Applying knowledge and understanding: to be able to foresee the use of these techniques to solve analytical problems, and to use these techniques in simple situations on commercial instruments. Capacity of autonomous judgement: to be able to apply the knowledge acquired to decide which is the optical technique to solve a given analytical problem. Communication abilities: to know how to present, in both oral and written forms, a problem and some possible solutions concerning the analysis of materials. Learning ability: to know how to gather information from textbooks and other material for the autonomous solution of problems concerning the analysis of materials.

Background of math, physics and chemistry.

Basics of optical spectroscopy – light-matter interaction – instrumentation used in optical spectroscopy – UV-vis absorption spectroscopy - UV-vis Fluorescence spectroscopy and microscopy - Fluroescence Nanoscopy – Vibrational spectroscopy (IR and Raman) and microscopy – IR and Raman nanoscopies – Surface-enhanced Raman Spectroscopy (SERS) Contents are in agreement with the teaching objectives of the course of studies.

AVV 2004 - Spectroscopic methods in mineralogy. Eds. Beran e Libowitzky. Eotvos University Press Brundle C.R., Evans Jr C.A., Wilson S. 1992 – Encyclopedia of materials characterization. Surfaces, interfaces, thin films. Butterworth-Heinemann Publishing. Hollas J.M. 2004 – Modern spectroscopy. John Wiley & Son, Inc. Jenkins R., Gould R.W., Gedcke D. 1995 – Quantitative X-ray spectrometry. Marcel Dekker Inc. Reed S.J.B. 2005 – Electron microprobe analysis and scanning electron microscopy in geology. Cambridge University Press. Skoog, West, Holler, Crouch "Fundamentals of Analytical Chemistry", Brooks/Cole, 2014 - ed. italiana "Chimica Analitica Strumentale" – Edises Valeur and Beberan-Santos "Molecular Fluorescence", Wiley-VCH, 2012 Larkin "IR and Raman Spectroscopy: Principles and Spectral Interpretation", Elsevier, 2011

Basics of optical spectroscopy –wave/particle duality for light – quantum behavior of matter - light-matter interaction – Jablonski diagram - instrumentation used in optical spectroscopy – sourced or radiation – diffraction gratings, prisms and monochromators – detectors - UV-vis absorption spectroscopy - selection rules - UV-vis Fluorescence spectroscopy and microscopy – lifetime and quantum yield –fluorescence anisotropy – FRET - Fluorescence Nanoscopy – Vibrational spectroscopy (IR and Raman) and microscopy – IR and Raman nanoscopies – Surface-enhanced Raman Spectroscopy (SERS)

Direct teaching with multimedia supports, classroom exercises.

No

Evaluation method: oral exam on the topics of the course, on both theoretical as well as applicative aspects. Evaluation criteria: examinations are aimed at ascertain the students’ knowledge of the topic of the course, as well as their ability to apply this knowledge. Marks are expressed as /30. The evaluations are expressed according to the following criteria: -Excellent (30 -30 laude): excellent knowledge of the topics, excellent language properties, excellent analytical skills; The student is able to brilliantly apply theoretical knowledge to concrete cases. -Very good (27 -29): good knowledge of topics, remarkable language properties, good analytical skills; The student is able to correctly apply theoretical knowledge to concrete cases. -Good (24-26): good knowledge of the main topics, good language properties; The student shows an adequate ability to apply theoretical knowledge to concrete cases. -Satisfactory (21-23): the student does not show full mastery of the topics main teaching, while possessing the fundamental knowledge; however, it shows satisfactory language properties and sufficient ability to apply theoretical knowledge to concrete cases. -Sufficient (18-20): minimum knowledge of the main topics of teaching and technical language, limited ability to adequately apply theoretical knowledge to concrete cases. -Insufficient (<18): the student does not have an acceptable knowledge of the contents of the different topics of the program.

This course concurs to the realization of the UN 2030 Agenda for Sustainable Development OBJECTYIVES 3,4,9